利用生物层干涉法进行快速高精度流感疫苗效价测定

在制造过程中快速准确地确定疫苗滴度在理解疫苗开发过程的性能以及正确缩放每个过程步骤中是重要的。

单个径向免疫区域(SRID)技术是疫苗滴度测定最常用的技术。然而,SRID是耗时的,并且通常表现出差的精度。因此,可以替代分析过程并提供在不同疫苗菌株上提供准确和精确效力数据的替代测定。

The Octet® platform’s Bio-Layer Interferometry (BLI) technology combines the high-throughput characteristics of a 96-well or 384-well plate format with improvements in precision and reproducibility and is derived from a simpler and more direct vaccine/antigen–antibody binding measurement method. They provide process development groups with a robust and easy to use alternative to the SRID method. BLI reduces the assay time from days to just a few hours for a 96-well plate of samples.



留话